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Primers used for quantitative PCR.
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Primers used for quantitative PCR.

Journal: International Journal of Medical Sciences

Article Title: Extracts of Portulaca oleracea and Patrinia scabiosaefolia relieve ultraviolet B-induced skin injury in solar dermatitis mice via inhibiting IL-17/CCL2 pathway and oxidative stress

doi: 10.7150/ijms.106289

Figure Lengend Snippet: Primers used for quantitative PCR.

Article Snippet: Mouse anti-mouse IL-17 and rabbit anti-mouse Act1 and CCL2 antibodies were obtained from Proteintech (Wuhan, China).

Techniques:

POPS extracts reduce macrophage infiltration and alleviate inflammation via inhibition of the IL-17/CCL2 pathway. (A) Representative images of F4/80 immunohistochemical staining of the skin of C57BL/6J mice 72 hours post-treatment. (B) Quantitative analysis of F4/80 immunohistochemical staining. (C) Western blot analysis of CCL2 protein expression levels in each group. (D) Quantitative analysis of the Western blot results for CCL2 (n=3 per group). (E) qRT‒PCR analysis of CCL2 mRNA expression levels in each group (n=3 per group). (F) Western blot analysis of IL-1β, IL-6, and TNF-α protein expression levels in each group. (G-I) Results from the quantitative analysis of the Western blot results for IL-1β, IL-6, and TNF-α (n=3 per group). (J-K) Quantitative analysis of collagen fiber abundance via Masson's trichrome staining. (L-N) Western blot analysis and quantitative assessment of MMP-2 and MMP-9 protein expression levels in each group. (O) Representative images illustrating the impact of macrophages on the level of inflammaging in fibroblasts following POPS intervention at various concentrations were obtained via SA-β-gal staining. (P) Quantitative analysis of SA-β-gal-positive cells measured by SA-β-gal staining. All data are presented as the means ± SDs. Statistical significance: ***p<0.001; **p<0.01; *p<0.05 vs. CON; ###p<0.001; ##p<0.01; #p<0.05 vs. MOD.

Journal: International Journal of Medical Sciences

Article Title: Extracts of Portulaca oleracea and Patrinia scabiosaefolia relieve ultraviolet B-induced skin injury in solar dermatitis mice via inhibiting IL-17/CCL2 pathway and oxidative stress

doi: 10.7150/ijms.106289

Figure Lengend Snippet: POPS extracts reduce macrophage infiltration and alleviate inflammation via inhibition of the IL-17/CCL2 pathway. (A) Representative images of F4/80 immunohistochemical staining of the skin of C57BL/6J mice 72 hours post-treatment. (B) Quantitative analysis of F4/80 immunohistochemical staining. (C) Western blot analysis of CCL2 protein expression levels in each group. (D) Quantitative analysis of the Western blot results for CCL2 (n=3 per group). (E) qRT‒PCR analysis of CCL2 mRNA expression levels in each group (n=3 per group). (F) Western blot analysis of IL-1β, IL-6, and TNF-α protein expression levels in each group. (G-I) Results from the quantitative analysis of the Western blot results for IL-1β, IL-6, and TNF-α (n=3 per group). (J-K) Quantitative analysis of collagen fiber abundance via Masson's trichrome staining. (L-N) Western blot analysis and quantitative assessment of MMP-2 and MMP-9 protein expression levels in each group. (O) Representative images illustrating the impact of macrophages on the level of inflammaging in fibroblasts following POPS intervention at various concentrations were obtained via SA-β-gal staining. (P) Quantitative analysis of SA-β-gal-positive cells measured by SA-β-gal staining. All data are presented as the means ± SDs. Statistical significance: ***p<0.001; **p<0.01; *p<0.05 vs. CON; ###p<0.001; ##p<0.01; #p<0.05 vs. MOD.

Article Snippet: Mouse anti-mouse IL-17 and rabbit anti-mouse Act1 and CCL2 antibodies were obtained from Proteintech (Wuhan, China).

Techniques: Inhibition, Immunohistochemical staining, Staining, Western Blot, Expressing

Journal: Cell Reports Medicine

Article Title: Genetic and microenvironmental evolution of colorectal liver metastases under chemotherapy

doi: 10.1016/j.xcrm.2024.101838

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-β-Actin (Cat# 60008-1-Ig, RRID: AB_2289225 ), mouse monoclonal anti-GAPDH (Cat# 60004–1-Ig, RRID: AB_2107436 ), mouse monoclonal anti-CCL2 (Cat# 66272-1-Ig, RRID: AB_2861337 ), rabbit anti-Ki-67 (Cat# 27309-1-AP, RRID: AB_2756525 ) and rabbit anti-CD31 (Cat# 11265-1-AP, RRID: AB_2299349 ) were obtained from Proteintech.

Techniques: Recombinant, DNA Sequencing, Mutagenesis, Software